The viral gag, pol, and env genes, necessary for particle formation and replication, are stably integrated into the genome of the packaging cell line.
而病毒颗粒识别、包装等过程所必须的gag、pol与env基因整合在相应的包装细胞基因组内。
参考来源 - 构建YMDD变异乙型肝炎病毒稳定表达细胞系的实验研究·2,447,543篇论文数据,部分数据来源于NoteExpress
MTT was used to detect the cell growth of the packaging cells.
MTT法检测加入反义寡核苷酸后对转染的包装细胞生长的影响。
Objective:To construct a recombinant defective retroviral vector carrying hNT3 gene and a stable virus-producing packaging cell lines , and then to identify them.
构建并鉴定携带人NT - 3基因的逆转录病毒载体以及稳定的包装细胞系。
Objective To develop a method for acquisition of efficient and stable retroviral packaging cells on the basis of fluorescence-activated cell sorting (FACS) technique.
目的探讨利用荧光激活细胞分选技术获得有效重组逆转录病毒包装细胞系的方法。
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