Long-overlapping PCR 大片断逐步步移PCR
overlapping-primer pcr 重叠引物pcr
overlapping extension pcr 重叠延伸pcr法
overlapping extention pcr 重叠延伸pcr
Aim:Rubella virus E1 gene was synthesized with overlapping PCR-Restriction Enzyme ligation method. Methods: Bioinformatics analysis of E1 gene was done and the E1 sequence containing Escherichia coli preferred-codons was designed.
目的:利用重叠PCR-酶切连接法人工合成风疹病毒E1基因的全长序列。
参考来源 - 重叠PCRThe supernate of virus culture was used as templet in overlapping PCR, then the interesting gene was obtained.
利用重组PCR的方法，以病毒培养液上清为模板，把二个目的基因串联在一起。
参考来源 - 重组人巨细胞病毒（HCMV）嵌合肽的构建及其在毕赤酵母中的表达·2,447,543篇论文数据，部分数据来源于NoteExpress
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