out off alignment
偏离对准
以上为机器翻译结果，长、整句建议使用 人工翻译 。
However, when carrying out systematic analysis the general multi-sequence alignment method obviously has its limitation, i. e. it cannot shake off its primary structure.
然而，在进行系统发育分析时通常的序列联配方法有明显的局限性，即无法摆脱一级结构的束缚。
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