...min、5min、10min、30min、60min时脑组织中的总RNA,经质量评价后,无降解的RNA进行一步法逆转录-聚合酶链式反应(one step RT-PCR),以GAPDH为内参照,通过针对Ngb mRNA、GAPDH mRNA的特异性引物进行扩增,对扩增后的产物进行1.5%的琼脂糖凝胶电泳,然后利用凝胶图像...
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one-step RT-PCR 一步法RTPCR ; 一步rt ; 一步法rt
GoldScript one-step RT-PCR kit 试剂盒
one step rt pcr 一步rt
Double one-step RT-PCR 双重一步法RT
BioRT One Step RT-PCR Kit BioRT逆转录扩增试剂盒
TITANIUM One-Step RT-PCR Kit 钛一step法RT
Conclusion: detection of BCR-ABL fusion gene by one step RT-PCR has excellent sensitivity and specificity. The method is simple and rapid, and is exactly used to clinic detection.
结论：一步法rt - P CR检测BCR - ABL融合基因有很好的灵敏度和特异性，操作简便、快速，特别适用于临床检测。
One step nested reverse transcriptase-polymerase chain reaction (RT-PCR) was used.
使用一步法巢式逆转录聚合酶链扩增法(RT - PCR)。
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