Computers were once isolated calculation engines.

最初，电脑是孤立的运算机器。

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Once isolated, the sheep are more easily made a meal of.

当羊群被分散、孤立的时候，就容易成为它的餐点了。

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It takes approximately five to six months for the first supplies of approved vaccine to become available once a new strain of influenza virus with pandemic potential is identified and isolated.

一旦鉴定并分离出具有大流行可能的流感病毒新毒株，大约需要5至6个月时间提供首批获批准的疫苗。

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But, part of the reason I think is, globalization is creating a global culture and the excitement and enthusiasm that we once saw in isolated cities, like Los Angeles, are spreading out and are seen more and more around the world.

不过,我认为原因之一是,全球化塑造了全球性的文化,原来只存在于单个城市,例如洛杉矶,的狂热与激情,扩散到了全世界各地

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This invited abuse from them, in much the same way that once isolated rhesus monkeys seemed to invite abuse from the colony.

NEWYORKER: Hellhole

But as the once-isolated island becomes more globally connected, its traditional diet faces greater competition.

NPR: Crete: Ancient Diet with Modern Message

His trip is the latest high-profile diplomatic visit to the once-isolated nation since a reformist government took office a year ago.

BBC: UN's Ban Ki-moon urges Burma to unite on path to change