脱靶效应(off-target effect)是目前CRISPR/Cas9面临的最大问题，即在基因组的非目标位置产生非必要的DNA突变。通俗来讲就是辨识错了需要编辑的目标基因。
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They created illusions of armies and battleships and masked the Suez Canal to such effect that confused Nazi bombers dropped their payloads off-target.
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