New class sign; Thesis teaching; Foothold textbook; Observation accumulation; Feeling life.

新课标；作文教学；立足课本；观察积累；感受生活。

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Detail is observation, success is gained by accumulation.

细节在于观察，成功在于积累。

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MAIN OUTCOME MEASURES: Morphological observation, determination of proliferation ratio, ALP stain, determination of matrix calcium accumulation and number of mineralized nodes with alizarin red stain.

主要观察指标：形态观察、增值率测定、碱性磷酸酶染色、细胞基质钙含量测定、茜素红染色显示矿化结节数。

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