VP1 nucleotide sequencing VP1核酸序列
nucleotide sequencing method 核酸序列测定法
partial nucleotide sequencing 部分序列分析
cloning and nucleotide sequencing 克隆及序列测定
sequencing of nucleotide 核苷酸序列分析
Nucleotide sequencing sequencing with end termination We detected C825T gene polymorphism with fluorescence PCR and MGB and conducted real-time PCR reaction with 4μl genome DNA in 40μl reaction volume.
1．2．4核酸序列测定采用末端终止法测序。 1．2．5实时荧光PCR检测C825T基因多态性取基因组DNA 4μl于40μl反应体积中进行Real-Time PCR反应。
参考来源 - GNB3基因C825T位多态性与肥胖相关性研究·2,447,543篇论文数据，部分数据来源于NoteExpress
Results The constructed vectors of EBO-WT and EBO-G87 were identified by restriction enzyme digestion and nucleotide sequencing.
结果构建的EBO G87和EBO WT重组载体经内切酶双酶切鉴定及核苷酸序列测定证实。
And identified HEV71 isolates were performed by gene amplification of VP1 coding region, nucleotide sequencing and homology analysis of evolution.
对分离到的HEV71阳性分离株进行VP1编码区基因扩增，核苷酸序列测定和同源进化分析。
Owing to the speed, easiness and accuracy of current nucleotide sequencing technology, it is likely to enable the reliable identification of sarcosaphagous flies.
该检测方法快速、 简便和精确，能作为法医鉴别食尸性苍蝇种类的可靠依据。
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