...白抗原的基因核苷酸序列有人设计了各血清型间共同和不同的引物,用套式聚合酶链反应(nestedpolymerasechainreaction,nested-PCR)检测Gilliam,Karp,kato健康搜索,kawasaki和kuroki五个血清型的相应基因,...
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nested pcr 巢式聚合酶链反应 ; 嵌套式PCR
RT-nested PCR 逆转录套式pcr
semi-nested PCR 半巢式聚合酶链反应 ; 半巢式PCR
Multiplex Nested PCR 多重巢式聚合酶链反应
nested PCR-SSP 序列特异引物
rt-nested-pcr 反转录套式pcr
triplex nested PCR 三重巢式PCR
half nested pcr 半套式pcr
nested pcr primer 嵌套式pcr引物
The titer of each recombinant virus were detected by magnifying method of fibroblast of little rat and PT-PCR and Nested-PCR. We compared with the two methods .
将各阳性克隆扩增培养,收获细胞上清,经超离将细胞上清浓缩,用小鼠成纤维细胞放大法和逆转录巢式PCR法测定各重组病毒的滴度,两者进行比较,结果表明,后者比前者操作简单、快速、灵敏。
参考来源 - 反义RNA抑制禽流感病毒PB1基因的研究·2,447,543篇论文数据,部分数据来源于NoteExpress
METHODS SENV-D and SENV-H DNA were detected in 191 cases who were divided into 6 groups with nested-PCR.
方法采用套式聚合酶链反应(n PCR)对191例、6种不同人群血清中SENV DNA进行D亚型和H亚型分析。
Objective To assess the feasibility of detection of HIV-1 proviral DNA on Dried Blood Spot (DBS) samples by nested-PCR assay.
目的研究干血斑(DBS)样本用于人类免疫缺陷病毒1型(HIV - 1)感染前病毒DNA基因诊断的可行性。
PCR, nested-PCR and digestion analysis of the Anti-TrxS gene from T1, T2 and T3 plants showed the foreign Anti-TrxS gene was inherited to the progenies.
对本研究所得转基因后代T1、T2和T3代进行了PCR、嵌套PCR以及嵌套PCR产物的酶切消化,结果表明目的基因完整的遗传给了后代。
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