These gene mutation distributing in all over the whole coding section of ATM gene. Every Exon exist the gene mutated site and no obvious hot mutated site be discovered.
这些突变分布于atm基因整个编码区，每个外显子都存在基因变异位点，没有发现明显的突变热点。
Methods The polyadenylation signal-deficient retrovirus vector mutated by PCR site-directed mutagenesis was used to make polyadenylation signal-deficient retroviruses by PA317 packaging cells.
方法使用人工定点突变多聚腺苷酸化信号的小鼠逆转录病毒载体，应用PA317病毒包装细胞获得多聚腺苷酸信号缺陷的重组逆转录病毒；
应用推荐