The mutant G138P was obtained by in vitro site directed mutagenesis of GI gene.
用双引物法对GI基因进行体外定点突变，构建了GI突变体g 138p。
Given the correct copy as a template, the recombination event occurred at the break site and 11 percent of the cells tested had traded one copy of the mutant gene for the correct version.
给予正确的版本作为模板，经检测，在断裂位点发生的重组事件中，11%的细胞用一个一个突变基因交换了一个正确的基因。
AIM: To establish the mutant of coding calcium binding fragment of the 13th exon of human thrombospondin-1 (TSP-1) gene with polymerase chain reaction (PCR) site directed mutagenesis technology.
目的：利用聚合酶链反应定点突变技术构建人血小板反应素1基因第13外显子编码钙结合域突变体。
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