With the developing of sequencing technology and increasing of isolating efficiency of the flanking sequence, construction of rice insertion-mutant library is an efficient method to study gene function?
随着测序技术的发展和旁邻序列分离效率的提高，构建水稻插入突变体库成为研究基因功能的有效方法。
参考来源 - 水稻突变体库的构建和旁邻序列分析In this paper, we screened salt tolerance mutants from two mutant libraries——activation tagging mutant library and estradiol induced activation tagging mutant library. The screening condition is 200 mmol/L NaCl.
本文对两个已有的拟南芥突变体库——激活标签突变体库和雌二醇诱导的突变体库进行耐盐突变体筛选,使用200 mmol/L NaCl作为筛选条件。
参考来源 - 过氧化氢预处理对拟南芥耐逆性的影响及拟南芥耐盐突变体的筛选·2,447,543篇论文数据，部分数据来源于NoteExpress
A strategy of shotgun antisense gene silencing was used to generate a rice mutant library.
尝试利用鸟枪反义基因沉默策略构建水稻突变体库。
The core technology in TILLING is screening mutant library by using single-strand endonuclease.
TILLING技术的核心是使用单链核酸内切酶大规模筛选突变体库。
Thus, it is suggested that enlarging the mutant library via tissue culture should be feasible in rice.
因此，利用组织培养的方法获得更多的突变体来构建突变体库是可行的。
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