The coding region of alkaline protease gene and its 3’ flanking terminator region from B. pumilus were subjected to random mutagenesis by error-prone PCR.
采用易错PCR方法对碱性蛋白酶基因编码区和终止子片段进行随机突变。
参考来源 - 脱毛蛋白酶产生菌的选育及其碱性蛋白酶基因的克隆与表达Based on the bioinformatics analysis and the crystal structure, the site-directed mutagenesis was used and 18 mutant vectors were gained.
因此我们在生物信息学和晶体结构的基础上设计了突变体。
参考来源 - Spindlin1的蛋白结构解析及相互作用蛋白的寻找·2,447,543篇论文数据，部分数据来源于NoteExpress
N the generation, usually intentional, of mutations 诱变 [genetics]
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