Objective to analyze the difference among the cell survival probability (s) results, which were measured and calculated with microscope, flow cytometry and MTT methods respectively.
目的分析倒置显微镜计数、流式细胞仪及MTT等三种方法检测放射线照射后细胞存活率(S)结果间的差异。
Methods Human microvascular endothelial cells(HMVEC) were cultured. MTT and number-calculating methods were used to observe cyclophosphamide on HMVEC′s proliferation and sprouts.
方法采用人微血管内皮细胞(HMVEC),用MTT和记数法观察不同浓度的环磷酰胺对HMVEC的增殖和出芽抑制活性。
Schwann cells were identified by immunohistochemistry with S-100 antibody and their proliferating function was assessed by MTT assay.
用s 10 0抗体鉴定所培养的细胞并用MTT法检测这些细胞的增殖能力。
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