1）混合酸酐法（mixed anhydride method）：也称氯甲酸异丁酯法。半抗原上的羧基在正丁胺存在下与氯甲酸异丁酯反应，形成混合酸酐的中间体，再与蛋白质的氨基反应，形成半...
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METHODS: The mixed anhydride method was used.
方法：用混合酸酐法合成。
The conjugates of DES-MCPE with proteins as complete antigens were prepared by the mixed anhydride method and the conjugation rate of them were analyzed by UV scanning.
以混合酸酐法将DES - MCPE与载体蛋白偶联形成完全抗原，并以紫外扫描分析了完全抗原的偶联比率。
The horseradish peroxidase (HRP) tagged antibody was prepared by means of modified NaIO 4 method and the HRP tagged hapten was prepared by means of mixed anhydride method.
以辣根过氧化物酶采用改良的过碘酸钠法标记抗体、混合酸酐法标记半抗原。
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