Methods Surgical specimens from 40 primary esophageal carcinoma were examined for VEGF expression and microvascular density by immunohistochemical staining.
方法对40例手术切除的原发性食管癌标本进行免疫组织化学染色，确定其VEGF的表达及微血管密度。
Methods The expression of TAF and microvascular density count in 80 osteosarcomas were investigated by immunohistochemical staining and morphometric technique.
方法：应用免疫组化和形态计量方法，检测80例骨肉瘤TAF表达和肿瘤微血管密度。
Methods The expression of VEGF and intratumoral microvascular density(MVD) in 80 osteosarcomas and osteosarcoma cell OS-732 was detected by immunohistochemical staining.
方法应用免疫组化和形态计量方法，检测80例骨肉瘤VEGF表达、肿瘤微血管密度（MVD），以及OS-732血管生成相关因子的表达。
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