The volume of enzymatic reaction was 300ul,including 0.03 mol/L the phosphate buffer solution,0.2 mol/L Li2SO4,0.01mol/L 2-mercaptoethanol,0.25mmol/L NADPH,,3.5 mmol/L DL-glyceric aldehyde,3mg AR. The temperature of rection was 35℃.
最终反应体系为总体积为300ul,各组分分别为:100ul PH6.2磷酸钾缓冲液(0.03 mol/L),30ul 2-巯基乙醇(0.01mol/L),30ul硫酸锂(0.2mol/L),18ul NADPH(0.25mmol/L),25ul DL-甘油醛(3.5 mmol/L),40ul(含粗酶约3mg),反应温度为35℃。
参考来源 - ARIs筛选模型的建立及相关药物的筛选·2,447,543篇论文数据,部分数据来源于NoteExpress
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