Methods The plasmid transfection mediated by liposomal transfection reagent, HPLC analysis and reverse transcription-PCR analysis were used in this study.
方法采用脂质体介导的质粒转染、流式细胞仪分析、逆转录- PCR及高效液相色谱(HPL C)分析等实验技术方法做有关分析研究。
Objective: To investigate the information of transgene integrations in mice mediated with lentiviral vectors, the inverse PCR was used to clone the sequences of integration sites.
目的：为分析慢病毒介导的转基因小鼠中外源基因整合位点的信息，应用反向PCR克隆整合位点序列。
CBF1 gene from rape was transformed into Arabidopsis thaliana by Agrobacterium tumefaciens-mediated method to screen its transgenic plants with resistance and conduct PCR detection and GUS staining.
采用根癌农杆菌介导法将油菜CBF1基因转入拟南芥，筛选抗性转基因拟南芥植株，进行PCR检测和GUS染色。
应用推荐