Methods Collagenase perfusion method and mechanical cutting method were used to isolate HSCs from rat fetal liver which were then cultivated by H-DMEM containing 10% fetal bovine serum.
方法：采用胶原酶灌注法及剪碎消化法分离大鼠胎肝干细胞，并用含10%优等胎牛血清的H - DMEM培养液培养。
The fiber formation regions are studied in mechanical chatter cutting method in which short metal fibers are machined.
本文研究了机械振动切削法制造金属短纤维的生成域。
Water jet, as a novel rock cutting tool, can be combined with mechanical method to increase penetrating efficiency.
水射流作为一种新型切割工具运用于石油钻井中，与机械联合破岩可提高破岩效率。
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