In this study,the ovary-drip method with higher transformation frequency and reproducibility was established for soybean. In addition,a marker-free and vector-free AlNHX1 gene cassette was transferred into soybean via ovary-drip method.
本研究优化了一种转化率较高、重复性较好的大豆子房滴注法,通过该方法将无载体骨架序列无选择标记的AINHX1基因元件导入大豆,提高了大豆的耐盐性,为其它功能基因在大豆转化中的应用奠定了基础。
参考来源 - 大豆子房滴注转化方法的研究及耐盐基因（AlNHX1）的转化In this study,the ovary-drip method with higher transformation frequency and reproducibility was established for soybean. In addition,a marker-free and vector-free AlNHX1 gene cassette was transferred into soybean via ovary-drip method.
本研究优化了一种转化率较高、重复性较好的大豆子房滴注法,通过该方法将无载体骨架序列无选择标记的AINHX1基因元件导入大豆,提高了大豆的耐盐性,为其它功能基因在大豆转化中的应用奠定了基础。
参考来源 - 大豆子房滴注转化方法的研究及耐盐基因（AlNHX1）的转化In order to solve these problems, many approaches have been developed to obtain marker-free transgenic plants (MFTPs), which has shown a tendency to eliminate any marker gene DNA sequences in the final product.
为了解决这些问题,近年来已有报道通过许多方法来获得无标记的转基因植物。 这种无标记的转基因技术最终将去除转化体中的标记基因序列。
参考来源 - 利用Cre/loxP系统的一个marker·2,447,543篇论文数据，部分数据来源于NoteExpress
We construct a marker-free vector of RB gene in order to apply with RB in the tomato late blight breeding and production.
为了使马铃薯抗晚疫病基因RB能够在番茄育种和生产中应用，构建了RB无选择标记载体。
Neither Barry nor De Jong, denied Tevez's support, was able to free himself from a spirited marker to receive, and both failed to cope with the industry and pressure of Pienaar, Cahill and Fellaini.
另一方面，巴里和德容尽管得到了特维斯的支持，依旧没有能够表现得像一个称职的防守者，他俩在与对方的皮纳尔、卡希尔和费莱尼的对抗中大败亏输。
The changes of related serum enzymes, liver-fibrosis marker, oxygen free radical and liver tissue pathology were observed after 8 weeks of treatment.
观察治疗8周后大鼠的相关血清酶类、肝纤维化标志物、氧自由基及肝组织病理学等变化。
应用推荐