Dominating methods in wheat gene cloning include homology-based cloning,map-based cloning,suppression subtractive hybridization,DDRT-PCR etc.
目前,小麦基因的克隆方法主要有同源克隆、图位克隆、差减杂交、差异显示等。
参考来源 - 六倍体小麦基因克隆方法研究进展Using map-based cloning technique,EMS30 has been mapped to a region of 96 kb between molecular markers F21M12 and F14N23 on chromosome 1.
采用图位克隆的方法对EMS30突变基因进行定位的结果显示,该基因位于拟南芥第一条染色体的分子标记F21M12和F14N23之间的96kb区间内,该区间包含25个基因。
参考来源 - 拟南芥白化突变体EMS30的基因定位与分析·2,447,543篇论文数据，部分数据来源于NoteExpress
The mutated gene was cloned by map-based cloning, and was shown to encode an SPP.
图位克隆表明一个编码SPP的基因发生突变。
This study provides a basis for map-based cloning and functional analysis of the target gene.
本研究为该基因的图位克隆及功能解析奠定了基础。
Abstract: the high-density markers are necessary for map-based cloning of rice genes, but the available marker could not reach the satisfying level.
摘要在水稻功能基因的图位克隆中，常需要高密度的分子标记，而目前公布的各种标记的密度还远未达到令人满意的程度。
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