Objective To test the expression of melanoma antigen-4 (MAGE-4) gene in human colonic adenocarcinoma tissue.

目的检测黑色素瘤抗原- 4 (MAGE - 4)基因在结肠腺癌中的表达情况。

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Analyze MAGE-4 antigen epitope by online predict software of protein secondary structure and amplify the epitope gene by PCR.

在线用蛋白质二级结构预测软件分析MAGE - 4抗原表位，PCR获取MAGE - 4抗原特征表位基因。

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Conclusion the prokaryotic expression vector for HSP70 and MAGE-4 epitope genes was successfully constructed, which provided a basis for the development of vaccine.

结论已成功构建了人hsp70与MAGE - 4抗原表位基因的原核表达载体，为疫苗研究提供了依据。

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