Long PCR 长片段PCR ; 长链pcr ; 长距离PCR
long pcr procedure 长片段pcr技术
long rt pcr 长链RTPCR
long-range PCR 长片段PCR ; 长片段pcr技术
long-distance pcr 长距pcr
long fragment PCR 长片段PCR
long distance-inverse PCR 长距离反向PCR
long chain pcr 长链pcr
long-distance-pcr 长距离pcr
Two large fragments of 5kb and 10kb are amplified by two-step Long-PCR,speculating its complete mitochondrial genome is about 15kb.
通过两步长PCR扩增,得到两个片段大小分别为约5kb和10kb,推测其基因组大小在15kb左右。
参考来源 - 中华卵索线虫和武昌罗索线虫线粒体基因组的研究·2,447,543篇论文数据，部分数据来源于NoteExpress
To understand intron 15 of human LDL receptor gene, the DNA fragments from exon 15 to exon 16 and the 3' end of intron 15 were amplified with long chain PCR and anchored PCR.
为了解人类LDL受体基因内含子15的遗传背景，利用长链PCR和锚定PCR分离了LDL受体基因外显子15-内含子15-外显子16和内含于15的3’末端片段。
In the meantime we tried other additive chemicals, such as DMSO, glycerin, formamide. They were no notable results in long PCR.
同时，我们发现其它的添加物，如DMSO，甘油，甲酰胺对长片段pcr的作用不明显。
Conclusion RNA can be extracted from the OCT-embedded long-stored tissues treated in 25% sucrose in PBS. And all the extracted RNA can be used to RT-PCR amplification.
结论从25%蔗糖磷酸缓冲液固定oct包埋的冰冻时间较长的组织标本中可提取组织rna，并用于进行RT - PCR扩增。
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