...术和限制性内切酶双酶切构建并鉴定EGFP-N1-hPDGF-B真核表达载体,体外培养兔成纤维细胞,传代培养后用阳离子脂质体(LipofectAMINE):EGFP-N1-hPDGF-B为3uL:1μg的比例转染,G418筛选获得稳定转染的细胞。
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通过阳离子脂质体(Lipofectamine2000,L2000)介导的细胞转染,并利用G418(Geneticin)筛选后,建立稳定高表达AQP5的SPC-A1和P...
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Lipofectamine Reagent 脂质体转染试剂
Fluoromicroscopy was applied to observe the transfectional effect after the plasmids enter CNE-2 cells via lipofectamine.
所得质粒经脂质体转染鼻咽癌CNE-2细胞,荧光显微镜观察转染效果。
参考来源 - 鼻咽癌基因治疗中miRNA重组质粒的构建及初筛·2,447,543篇论文数据,部分数据来源于NoteExpress
以上来源于: WordNet
Conclusion We have successfully transfected HBV into HepG2 cells via lipofectamine and established HBV replication models in hepatoma cells.
结论通过脂质体介导法将HBV全基因转染至肝癌细胞,成功建立了HBV复制状态的细胞模型。
ConclusionUsing GFP as a report gene in eukaryotic cell transfection, the lipofectamine method could get a higher transfection efficiency and higher survival rate for cells.
结论采用绿色荧光蛋白为报告基因的真核细胞转染技术中,脂质体法是效率高、安全性大的方法。
CONCLUSION Lipofectamine may be used as a tool to enhance the efficacy of transfection with recombinant adenovirus.
结论可以利用阳离子脂质体来提高重组腺病毒的转染效率。
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