Methods:Viral RNA Was extracted from feces. 92 samples were detected by real-time fluorescent quantitative PCR and Enzyme-linked Immunosorbent Assay(ELISA).
方法：提取粪便中的RNA，用实时荧光PCR方法对丽水市急性胃肠炎的92份标本进行检测，并与常规ELISA检测结果比较。
Methods Hepatitis B serum markers and Pre-S2 antigens were tested by enzyme-linked immunosorbent assay and HBV DNA was detected by fluorescent quantitative-PCR in 982 hepatitis B patients.
方法 用酶联免疫吸附试验对982例乙肝患者血清标志物和乙肝病毒前S2抗原进行检测；并用荧光定量PCR法对其进行HBV-DNA检测。
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