The recombinant plasmid was transferred into Agrobacterium tumefaciens GV3101 respectively using the freezethaw method. Transgenic tobacco plants recovered from leaf discs applying Agrobacterium mediated gene were selected on solid medium containing Kanamycin.
重组质粒通过冻融法转化根癌农杆菌GV3101,利用农杆菌介导的叶盘法转化模式植物烟草。
参考来源 - 产肠毒素大肠杆菌K88·2,447,543篇论文数据,部分数据来源于NoteExpress
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