Methods The L and M segment cDNA of Hantavirus Q32 strain was amplified by RT-PCR. The purified PCR products were sequenced directly or cloned into pGEM-T Vector and then sequenced.

方法设计特异的PCR引物，用RT-PCR技术分段扩增Q32株全长L、M片段，PCR产物纯化后直接测序，或用T-A克隆方法进行PCR产物克隆，然后进行遗传进化分析。

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Quincy Jones and Lionel Richie co-produced the single, and,with a production team that included rappers Will.i.am, Kanye West and L.L.Cool J, a new music segment and lyrics were added.

VOA: standard.2010.02.18

In the Savings and Loan crisis it was a certain segment of the banking community--the S&L's that seemed to be in trouble but it didn't seem to be then a house of cards that could spread around the country and around the world.

在储贷危机中，只是银行机构中的,一个特定部分-储蓄和存款,陷入了困境,但是这似乎不是一个,能够延伸至整个国家,和整个世界的不切实际的无法实现的计划。

耶鲁公开课 - 金融市场课程节选

My favorite filler segment, of course, was the mashup of everyone's favorite sugarplum fairy L.A. Reid's incredible seated dance moves.

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