The materials were from Japanese flouner gynogenesis families. 14 pairs of primer were designed according to Japanese flounder growth hormone gene sequence, in which six pairs of could be useful and the total of 1736bp sequence was amplified.
以牙鲆雌核发育家系为材料,根据牙鲆生长激素基因序列设计了14对引物,其中6对引物扩增可用,共扩增出总长1736bp的序列。
参考来源 - 牙鲆选育家系生长性状的遗传分析In addition, under the experiment conditions, dietary vitamin E and n-3 HUFA had synergistically effect on non-specific immune response and disease resistance of Japanese flounder (P<0.05).
此外在本实验条件下，饲料中维生素E和n一3 HUFA对牙虾非特异性免疫力和对爱德华氏菌疾病抵抗力有明显的协同作用(P<0.05)。
参考来源 - 免疫增强剂对牙鲆（Paralichthys Olivaceu）免疫力和抗病力的影响·2,447,543篇论文数据，部分数据来源于NoteExpress
Myostatin(MSTN)gene was isolated from Japanese flounder(Paralichthys olivaceus)by homology cloning strategy and genome walking.
采用同源克隆及基因组步移的方法，分离克隆了牙鲆肌肉生长抑制素（MSTN）基因。
It is a basic work for the application of marker-assisted selection and cloning of anti-disease gene in Japanese flounder selective breeding program.
这些抗病性候选标记的获得为实现牙鲆分子标记辅助育种和抗病基因克隆奠定了一定基础。
With the expansion of the size of the Marine fish farming, the breed of Japanese flounder fish and the research on the development of its feeding have also developed.
随着海水鱼养殖规模的不断扩大，对鲆鲽鱼类养殖以及其饲料开发的研究也随之增多。
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