...已被美国FDA批准为安全的基因工程受体生物。在构建重组工程菌的过程中,通常会采用操纵子来控制外源基因的可控表达。异丙基硫代半乳糖苷(IPTG)是原核表达系统中最常用的高效诱导剂,能够稳定地启动乳糖操纵子,使目的基因得到持续高效的表达。
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IPTG induction IPTG诱导
iptg诱导 iptg induction
IPTG concentration IPTG浓度
iptg inducing iptg诱导
induced by iptg iptg诱导
induced with iptg iptg诱导
induction by iptg iptg诱导
concentration of iptg iptg浓度
The mutant was overexpressed in Escherichia coli BL21(DE3) by IPTG induction.
IPTG诱导突变体在大肠杆菌BL21(DE3)中高效表达。
The modified gene was expressed by IPTG induction and purified by affinity chromatography and cleavage in place.
IPTG诱导该基因的表达,亲和层析和原位裂解法纯化表达产物。
After optimizing prokaryotic expression conditions, we determined the optimum inducement time and concentration of IPTG.
通过优化原核表达条件,确定了原核表达的最佳诱导时间和诱导剂浓度。
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