If I cut both the plasmid and my DNA of interest with the same restriction enzyme I'm going to end up with the same sticky ends on both molecules.

如果用同一种限制性内切酶,来切割质粒和我感兴趣的DNA,在两个分子上就能得到同样的粘性末端

耶鲁公开课 - 生物医学工程探索课程节选

Behind each constitutional restriction stands a vested interest of one sort or another.

ECONOMIST: Brazil

The details are under review: foreigners may be allowed to buy a bit more without restriction, and still more if the government thinks it is in the national interest.

ECONOMIST: First they went for the currency, now for the land