Pratylenchus coffea was monoxenically reproduced on carrot disk culture, and the effect of time, temperature, and inoculum density on reproduction were discussed in the study.
利用愈伤组织法对咖啡短体线虫进行了人工繁殖，并分析讨论了温度、培养时间、接种量对繁殖量的影响。
The result indicates: under 30% conditions, the production effect of the sunflower plate after the apple marc pretreatment's powdery in the inoculum density is better ; 3.
结果表明：经过果汁灌底物预处理的粉末状葵盘在接种物浓度为30%的条件下产气效果更好；
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