The proper low-temperature pretreatment can improve the initiation rate;
适当的低温预处理可以不同程度提高诱导率；
The secondary structure of the translation initiation region (TIR) is a determinant factor for translation initiation rate, meanwhile, codon preference plays an important role, too.
翻译起始区(TIR)的二级结构是影响翻译效率的决定性因素，同时密码子的偏好性问题也是个至关重要的方面。
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