As ligands, L-tryptophan and synthesized polypeptide of AChR at residues 67~76 were immobilized on cellulose beads to make immunoadsorbent. The influence of immobilizing conditions on immobilizing quantity was observed in detail.
将L-色氨酸、人工合成的人AChRα亚单位67~76位10氨基酸多肽作为配基固定于环氧氯丙烷活化后的球型纤维素上制备重症肌无力免疫吸附剂,并详细研究了固定化条件对固定量的影响。
参考来源 - 重症肌无力特异性免疫吸附剂的制备及对患者血清的静态吸附试验·2,447,543篇论文数据,部分数据来源于NoteExpress
CONCLUSION: IgE immunoadsorbent is successfully prepared through cross-linking, activation of AGAR gel beads and immobilization of tryptophan ligand.
结论:以琼脂凝胶微球载体,通过交联、活化和固定配基色氨酸成功制备免疫球蛋白e免疫吸附剂。
The reactivity of these recombinant proteins with sera from patients with amebiasis was examined by means of enzyme-linked immunoadsorbent assay (ELISA).
所获得的重组蛋白,用ELISA法检测它们与阿米巴病患者血清的反应性。
应用推荐