本文将含有潮霉素抗性(Hygromycin Resistance)的载体质粒pBHtl通过冻融法导入根癌农杆菌AGL-1中,与H5孢子共培养两天后筛选得到具有潮霉素抗性的转化子。
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hygromycin resistance gene 潮霉素抗性基因
hygromycin B resistance 潮霉素抗性
hygromycin b resistance gene 抗潮霉素b基因
Mendelian inheritance of hygromycin resistance was showed as single dominant locus and proved by Southern blotting analysis in T1 and T2 generation of all transgenic plants.
T1和T2代中潮霉素抗性表现为显性单基因位点的遗传方式，符合孟德尔分离规律，并得到分子鉴定结果的证实。
The resistance concentration of hygromycin selected is the basis for testing transgenes millet plant.
潮霉素抗性浓度的筛选为检测谷子转几丁质酶基因奠定基础。
The regenerated fertile plants were obtained by effective selection for hygromycin B resistance.
幼胚诱导的愈伤组织，经潮霉素抗性筛选，得到可育的再生植株。
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