Methods Human umbilical vein endothelial cells (HUVECs) were cultured in vitro.

方法体外培养人脐静脉内皮细胞，通过传代形成细胞的衰老模型。

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The changes in cell cycle progression of synchronized HUVECs were examined by flow cytometric analysis.

用流式细胞技术检测各组细胞周期，观察细胞增殖能力的变化。

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The cultured HUVECs were divided into 6 groups according to different experimental agents: control ( serum - free DMEM ) ;

施加实验因素，分成6组：正常对照组（无血清DMEM）；

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