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NIH 3T3 cells were transfected by human papillomavirus 18(HPV 18). HPV 18 DNA as donor and calf thymus DNA as vector. Calcium phosphate technique was used.
以HPV-18 DNA 为供体、小牛胸脉DNA 为载体，NIH 3T3细胞为受体细胞，应用磷酸钙沉淀法将供体DNA 引入细胞内，得到了清晰的NIH 3T3细胞形态学转化灶。
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