Purpose to detect P63, K3 expression in human limbus, to approach the possibility of P63 acted as the marker of human limbal stem cells.
目的通过检测人角膜缘组织中P 63、K3蛋白表达的情况,探讨P 63蛋白作为角膜缘干细胞阳性标记的可能。
Objective To construct the model of human limbal stem cells culture in vitro and evaluate the effect of autologous limbal stem cells transplantation treating with unilateral ocular surface disease.
目的:建立人角膜缘干细胞体外培养模型; 探讨单眼角膜缘干细胞缺陷患者自体角膜缘干细胞体外培养后再移植的临床疗效。
Method:Autologous or allosome limbal stem cells and human amnion were used in 48 patients with severe chemical injury, eyeball adhere, recurrent pterygium.
方法:采用自体或异体角膜缘干细胞联合保存人羊膜移植治疗重症化学伤、眼球粘连、复发性胬肉。
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