METHODS: To carry out qualitative and quantitative analysis by means of HPLC DAD detection, heuristic evolving latent projections (HELP) resolution method and overall volume integration method.

方法：用HPLCDAD联用色谱检测，借助HELP法解析，基于组分的色谱保留时间和光谱曲线进行定性，然后用总体积积分法定量。

youdao

Aim: to study the feasibility and influential factors for the resolution of HPLC-DAD data of chiral drugs by non-negative matrix factorization (NMF) algorithm.

目的：研究非负矩阵因子分解算法(NMF)用于手性药物HPLC - DAD二维数据解析的可行性及其影响因素。

youdao

Then the pretreated samples were extracted with acetonitrile, further cleaned up by reverse solid phase dispersion, and analyzed by reversed phase HPLC with diode array detector (DAD).

韭菜样本用微波炉加热处理，使酶钝化消除含硫基质干扰，然后用乙腈提取、逆固相分散净化，用反相高效液相色谱-二极管阵列检测器检测。

youdao