99年5月9日来我所就诊，只用药两个半月，乙肝病毒（HBV-PCR）转阴了，接着又服药进行巩固，于2000年3月和2001年的8月两次追访再进行复查时，二年多过去了（HBV-PCR）仍为阴性。
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Methods an Alkaline lysis method was used to isolate serum HBV-DNA for fluorescence quantitative PCR and compared with conventional phenol-chloroform extraction method and boiling lysis method.
方法使用碱裂解法、酚氯仿法和煮沸裂解法同时提取血清hbv - DNA，比较荧光定量pcr测定的重复性和测定值的差异。
HBV gene was examined in the genome of DNAs from larvae of housefly by PCR.
背景与目的：探讨人乙肝病毒基因能否整合在家蝇基因组中。
Methods HBV genotypes were detected by polymerase chain reaction (PCR) and restriction fragment length polymorphism analysis (RFLP) in 136 HBV DNA positive patients who were born in Shanghai.
方法采用聚合酶链反应-限制性片段长度多态性分析(PCR - RFLP)检测136例HBV DNA阳性的上海籍HBV感染者的基因型。
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