病毒基因组大部分为编码序列； 噬菌体ΦX174（phage phix 174） 基因重叠 （Gene overlap） 环状单链DNA 利用有限的核 酸贮存更多的 遗传信息，提 高自身在进化 过程中的适应 能力。
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Overlapped gene expression. It borrows the idea of overlap gene expression from biological study, introduces overlapped gene expression, and saves space for gene expression.
重叠基因表达，借鉴生物基因片段重叠表达，引入重叠基因概念，节约了表达空间；
METHODS IL_3 signal sequence and human endostatin gene were amplified with PCR and spliced with overlap extension PCR.
方法用PCR方法扩增il_3信号肽序列和人内皮抑素基因，然后采用重叠延伸pcr拼接的方法将两者拼接和扩增。
The overlap extension mediated by restriction endonuclease to obtain the full length gene was established.
建立一种用于克隆全长基因的、限制性内切酶介导的重叠延伸法 。
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