扩增我国登革2型病毒全长cDNA分子的融合PCR方法_医网打尽文献搜索 关键词：登革热病毒;全长cDNA;长链逆转录PCR;融合PCR [gap=1783]Keywords：dengue virus; full-length cDNA; long RT-PCR; fusion PCR
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Fusion-PCR 融合PCR
RT-PCR was successful in 92% of tested fibrolamellar carcinoma cases (24/26) and the DNAJB1-PRKACA fusion transcript was found in all fibrolamellar carcinomas but not in other tumor types.
RT-PCR在92%的纤维板层型肝细胞癌病例（24/26）中是检测成功的，并在所有成功纤维板层型肝细胞癌中发现了DNAJB1-PRKACA融合转录体，而其它类型的肿瘤均未发现。
Conclusion: detection of BCR-ABL fusion gene by one step RT-PCR has excellent sensitivity and specificity. The method is simple and rapid, and is exactly used to clinic detection.
结论：一步法rt - P CR检测BCR - ABL融合基因有很好的灵敏度和特异性，操作简便、快速，特别适用于临床检测。
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