Objective Adopt two methods to produce cells of feeder layer and compare and observe its result of culture to find a better method of culture.
目的采用两种方法制备饲养层细胞，对比观察培养效果，求得最佳培养方法，以期实现其有效利用。
Conclusion Serum-free and feeder layer-free culture seems to be an ideal method for the cultivation of HKCs.
结论无血清、无滋养层培养，是一种较理想的培养HKC的方法。
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