The recombinant plasmids containing mutant genes were transformed into the Escherichia coli strain BL21 (DE3), and the expressed proteins were found to be water soluble after the induction of IPTG.
将含突变基因的重组质粒转化大肠杆菌菌株bl2 1 (DE3),经IPTG诱导表达获得的目的蛋白质均以可溶形式存在。
Objective To construct the prokaryotic expression vector of the sporozoite surface antigen gene of Eimeria tenella GZ strain and expression in Escherichia coli.
目的构建柔嫩艾美耳球虫子孢子表面抗原原核表达载体,并且在大肠杆菌中表达。
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