...B(Oxford Immunotec,Abingdon,UK)试剂盒检测外周静脉血淋巴细胞对于2种结核分枝杆菌菌种特异性分泌蛋白:早期分泌靶抗原6(ESAT-6)和培养分泌蛋白10(CFP-10)的免疫反应。
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酶联免疫斑点检测对结核性胸膜炎诊断价值的比较研究-期刊文摘-论文中国 此方法灵敏稳定,已被越来越广泛的应用于医学研究中。其在结核病诊断方面主要集中在应用结核分枝杆菌RD1区编码的早期分泌抗原靶(early secreting antigen target 6KD ,ESAT-6)和培养滤液蛋白(culture filtrate protein 10KD ,CFP-10)及其多肽
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Conclusion: The ESAT-6 fusion protein was successfully expressed and purified, identified.
结论：成功表达纯化并鉴定构建了ESAT - 6融合蛋白。
Results Compared with the amino acid sequence of ESAT-6 of Mycobacterium tuberculosis published, the homologies was 90%.
结果结核分支杆菌重组抗原esat - 6与已发表氨基酸序列同源性为90%。
To construct the recombinant prokaryotic expression vector containing the ESAT-6 gene of Mycobacterium Boris, purification, expression the fusion protein identified.
构建牛结核分枝杆菌esat - 6基因的原核表达载体，诱导表达、纯化并初步鉴定该蛋白。
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