Next, using FETAX test, tissue section and whole mount in situ hybridization technique to study the teratogenic effect of three herbicides on early stages of development of X. tropicalis.
接下来利用爪蟾胚胎致畸实验（FETAX）、组织切片技术和整体原位杂交技术，研究了三种酰胺类除草剂对热带爪蟾胚胎的致畸效应。
Methods Embryonic to aged stage murine embryo and brain sections were stained by whole-mount in situ hybridization technique.
方法采用胚胎期至老年期小鼠全胚胎及脑切片的原位杂交染色。
The key factor of the success to mouse whole mount RNA in situ hybridization is whether the proteinase K treatment conditions are optimal or not.
蛋白酶K处理条件是否适当是小鼠全胚胎RNA原位杂交成功的关键因素。
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