Objective; To construct a promoter identifying plasmid with GFP as reporter gene, and then identify the promoter activity of HCV 5 '- UTR with this construct.
目的：利用已有质粒构建带GF P报告基因的启动子鉴定质粒，并用此质粒鉴定丙型肝炎病毒5非翻译区(HCV 5 ' - UTR)启动基因表达的活性。
The sequencing of highly conserved 5' UTR of the Chinese HGV strain has important implication for the development of genetic diagnostic assays.
HGV高度保守区5＇UTR的分子克隆与核苷酸序列的测定对开展HGV感染的基因诊断具有重要意义。
Results it was found by sequence contrast that some mutation sites occurred mainly on UTR region, and a lot of protein sequences changed according to changes of genes coding region.
结果经序列比对发现突变位点较多发生在U TR区域，同时一些基因的编码区域也发生蛋白序列的变化。
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