Objective To construct a bait plasmid in bacterial two-hybrid system.

目的构建大肠杆菌双杂交系统的诱饵重组质粒。

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CONCLUSION: Yeast two-hybrid GAL4 system 3 can be utilized to fish HCV core region-interacting protein. HCV core protein does not have self-activating function.

结论利用酵母双杂交Gal4系统3研究与HCV核心蛋白相互作用的蛋白，证实了HCV核心蛋白无自激活功能。

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Nevertheless, huge protein network is larger than that we predict and single yeast two-hybrid system cannot solve all the problems, which need be complemented by other wags.

但是巨大的蛋白质网络比我们想象要大得多，单一的双杂交系统不能解决所有问题，需要同其它的方法有效地结合。

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Its current two-mode hybrid system is limited to large trucks and SUVs, like the Chevy Tahoe and Cadillac Escalade.

FORBES: Magazine Article

The first GM-designed and built electric motors are scheduled to debut in 2013 in its next-generation, rear-wheel-drive, two-mode hybrid technology.

FORBES: Magazine Article

C-MAX Energi operates just like a traditional hybrid with two key differences - owners must plug in the vehicle using its charge port and C-MAX Energi has a larger battery pack.

ENGADGET: Ford's 2013 C-Max Energi rolls out 95 MPGe, arrives later this fall