Methods (1) The coding sequence of MG7 mimotope (GC23) was included in the reverse primer, and by PCR incorporated with HSP70 gene at the 3 terminus.
方法1。根据HSP70全长基因序列设计针对其编码区的引物，在下游引物的宁端加人MG7模拟表位的核昔酸序列。
Its RNA polymerase activity was dependent upon the primer in vitro with limited reverse transcription activity.
其RNA依赖的RNA聚合酶活性有一定的引物依赖性。
A TC-RT-PCR method basing on reverse transcription with random primer facilitated the detection for those samples mixed infected by ASGV and ACLSV.
用随机引物反转录，通过TC-RT-PCR检测ACLSV和ASGV复合感染的梨样品也均获得了目标片段。
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