降落PCR扩增人类常染色体STRD15S128 关键词] D15S128;降落PCR;退火温度;PCR特异性 [gap=616]Key words] D15S128;TD-PCR;Annealing temperature;PCR specificity
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The detection rates of TD-PCR for clinical samples were the same as that of ELISA (100%), and higher than that of viral isolation (75%).
运用TD - P CR、ELISA以及病毒分离对临床病料进行检测，TD - PCR方法与ELISA检测结果一致(100%)，均比病毒分离检出率(75%)高。
Methods Single round Touchdown PCR (TD PCR) and restriction fragment length polymorphism (RFLP) method were used for genotyping in 110 people from the Han population in Chongqing.
方法对110名重庆汉族个体，采用降落式PCR和限制性片段长度多态性法（RFLP）进行基因型分析。
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