Results the higher concentration of the protein of each sample and the better 2-de profile of each sample protein were obtained by the three-step method than by the single-step method.

结果：三步法提取的宫颈癌组织蛋白质含量较一步法有明显的增高，2 - DE蛋白质图谱较一步法清晰、分辨率高。

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The ideal, for plan time, step by step, improve weaknesses, toast and since Mr, in retrospect, combining exertion, seek the backing, concentration, all is the method of working.

追求理想计划时间，一步一步改进弱点，举杯自贺，回顾往事，劳逸结合，寻求后盾，专心致志，都是巧干的方法。

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